SIWES Report: In the course of my industrial training at Onmed Diagnostic Laboratories, I worked in four departments. The departments include Haematology department where all the tests involving blood are done including cross matching test that precede blood transfusion, Parasitology department where parasitic infections are investigated, Microbiology department where micro-organisms are cultured and sensitivity test being done including other serological tests, Clinical chemistry department where test that assist in disease diagnosis and prognosis, monitoring treatment and screening to prevent ill health.
In each of the departments, certain rules and regulations are adhered to in order to obtain reliable results from any experiment being carried out. Specimens for the tests are being collected in appropriate specimen containers. A correct procedure for each test is followed strictly and the result is recorded immediately as soon as it is ready. Amongst the tests I was introduced to, are elaborated in this work
BASIC LABORATORY PROCEDURES-SIWES Report
Certain laboratory procedure ranging from handling specimen to obtaining results have to strictly be observed and kept if accurate, real and reliable results are to be obtained
COLLECTION OF SPECIMEN :-SIWES Report
BLOOD SPECIMEN : This is done by tieing the tourniquet around the upper arm in order to make the patient’s vein prominent. Clean the portion from where the needle is to be injected with a spirit swab. Inject the needle into the vein and collect the amount of blood that is needed for the test. Untie the tourniquet before pulling the needle. The specimen collected is put into the appropriate anticoagulant eg ethylene diamine tetra acetic acid(EDTA) for blood grouping, genotyping, widal and malaria parasite tests etc, fluoride oxalate for FBS, RBS. Blood sample can also be put in a tube without anticoagulant if serum is required.
STOOL SPECIMEN : This is collected in a disposable sterilized container
URINE SPECIMEN : This is collected in a sterilized bottle should culture be needed
SPUTUM SPECIMEN : This is also collected in a disposable sterilized container.
Ear, eyes, urethral, virginal and throat swabs are collected using swab sticks.
SOME HEALTH AND SAFETY RULES IN THE LABORATORY-SIWES Report
- Laboratory coat must be worn at all times by the laboratory personnel within the laboratory.
- Eating, drinking and application of make-ups is highly restricted
- Work benches should be cleaned with disinfectant before and after everyday work.
- Hand gloves should be worn at all working times and removed when not at work. They should not be worn outside the laboratory.
- All samples collected must be labelled immediately to avoid misplacement.
- The laboratory personnel should not talk when culturing to avoid contamination of culture plates.
- Needles should be recapped immediately after used to collect blood.
- Non-disposable specimen containers including slides should be soaked and washed with disinfectant and rinsed with clean water.
- When using non-disposable stirrer (mostly for serological works) clean the tip very well before using it for another sample.
- All results of the tests done are recorded immediately on the record book. Mental capacity should not be relied on.
Author: Okoye Chibueze Michael
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